Norcantharidin (NCTD) is the demethylated form of cantharidin, which is the active substance of mylabris, and is known to\r\nhave anticancer potentials. The aim of this paper was to assess the apoptosis-inducing effect of NCTD on HL-60 cells. Methods.\r\nThe effects of NCTD were detected by flow cytometer on the cell toxicity, cell cycle, and apoptosis of HL-60 cells cultured in\r\nvitro. Results. After 48-hour treatment with NCTD, the growth of HL-60 cells was inhibited significantly. The summit of apoptosis\r\nappeared after 24 hours. The percentage of the cells in G1 phase decreased and then increased in S and G2 M phase, while the S\r\nand G2 M phases were blocked after treatment with 5, 10, and 50 �µmol/L NCTD for 24 hours. Conclusions. NCTD can induce\r\nthe apoptosis of HL-60 cells and inhibit the fissiparism, and the domino effect was obviously correlated with the time and dosage.
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